SOLIDEX^®-ISOEx Набор для изоляции неимпregnated человеческих пан-T-клеток (без колонок)

Superior Isolation of High-Purity CD3+ T Cells from Human PBMCs Using SOLIDEX^®-ISOEx Untouched Human Pan-T Cell Isolation Kit (Column-free)

Catalog No. GM-Pan-T-Cell-iso-kit-CF
To evaluate the isolation efficiency of the SOLIDEX^®-ISOEx Untouched Human Pan-T Cell Isolation Kit (Column-free), CD3+ T cells were enriched from human peripheral blood mononuclear cells (hPBMCs) via negative selection. In this column-free protocol, non-target cells were labeled with a biotinylated antibody cocktail followed by magnetic beads. The labeled cells were then sequestered by a magnetic separator, allowing the highly purified, untouched CD3+ T cells to be recovered directly from the supernatant. Cell fractions were stained with CD56-PE and CD3-APC for flow cytometric analysis. The frequency of CD3+ T cells demonstrated a substantial increase from 40.7% in the pre-isolation sample to 92.5% in the post-isolation fraction. These results confirm that the column-free system provides effective depletion of non-target populations, yielding high-purity CD3+ T cells while maintaining their native state and maximal viability.

Figure 21. Rapid isolation and high-purity enrichment of Human CD3+ T Cells Using SOLIDEX^®-ISOEx Untouched Human Pan-T Cell Isolation Kit (Column-free).Representative flow cytometry plots showing the frequency of CD3+ T cells in hPBMCs before isolation (40.7%) and after enrichment via column-free negative selection (92.5%).