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SOLIDEX®-ISOEx Untouched Human Classical Monocyte Isolation Kit (Column-based)

🏷 GM-Tg-hg-T23212-iso-kit-1
IndirectColumn-basedNon-releasable
Isolate pristine Human Classical Monocytes (CD14+CD16-) with the SOLIDEX®-ISOEx Kit. Utilizing a superior Column-based Negative (Untouched) selection strategy, this kit depletes non-target cells from PBMC using optimized Magnetic Beads. The eluted cells are free of antibodies and beads, ensuring Zero Activation and preserving their native phenotype. Achieve High Purity and high viability for sensitive downstream applications like macrophage differentiation.
SOLIDEX®-ISOEx Untouched Human Classical Monocyte Cell Isolation Kit (Column-based) is a Indirect CD14+ Cell Isolation Kit (Column-based, Non-releasable)
📄 MSDS

Информация для заказа

Каталожный № Название продукта Размер Цена (RUB)
GM-Tg-hg-T23212-iso-kit-1-10T SOLIDEX®-ISOEx Untouched Human Classical Monocyte Isolation Kit (Column-based) 10 Tests RUB 17,860.00
GM-Tg-hg-T23212-iso-kit-1-50T SOLIDEX®-ISOEx Untouched Human Classical Monocyte Isolation Kit (Column-based) 50 Tests RUB 53,580.00
GM-Tg-hg-T23212-iso-kit-1-100T SOLIDEX®-ISOEx Untouched Human Classical Monocyte Isolation Kit (Column-based) 100 Tests RUB 94,376.00
GM-Tg-hg-T23212-iso-kit-1-750T SOLIDEX®-ISOEx Untouched Human Classical Monocyte Isolation Kit (Column-based) 750 Tests
SOLIDEX®-ISOEx Untouched Human Classical Monocyte Isolation Kit (Column-based)

Характеристики

  • Целевая популяция клеток CD14+CD16- Cell
  • Тип маркировки Indirect
  • Тип гранул Non-releasable
  • Формат Column-based
  • Метод выделения Negative Classical Monocyte Isolation (Untouched)
  • Хранение Хранить при 2℃ - 8℃

Описание

Каталожный № GM-Tg-hg-T23212-iso-kit-1
Название продукта SOLIDEX®-ISOEx Untouched Human Classical Monocyte Isolation Kit (Column-based)
Английское название SOLIDEX®-ISOEx Untouched Human Classical Monocyte Isolation Kit (Column-based)
Описание SOLIDEX®-ISOEx Untouched Human Classical Monocyte Cell Isolation Kit (Column-based) is a Indirect CD14+ Cell Isolation Kit (Column-based, Non-releasable)
Применение SOLIDEX®-ISOEx Untouched Human Classical Monocyte Cell Isolation Kit (Column-based) is used for Negative CD14+ Cell Isolation (Untouched), with the Key Features, such as Preserves native cell function; maintains surface marker integrity; ideal for functional studies
Компоненты
  • GM-Tg-hg-T23212-iso-Cocktail-1-50μL/250μL/500μL/3.75mL: -50μL/250μL/500μL/3.75mL
  • GMP-SMT-219-Ab01-nanoIMB-300μL/1.5ml/3ml/22.5ml: SOLIDEX®-ISOEx anti-Biotin Nanobeads (Column-Based)-300μL/1.5ml/3ml/22.5ml
Тип маркировки Indirect
Тип гранул Non-releasable
Формат Column-based
Ключевая особенность Preserves native cell function; maintains surface marker integrity; ideal for functional studies
Целевая популяция клеток CD14+CD16- Cell
Хранение Хранить при 2℃ - 8℃

Применение

SOLIDEX®-ISOEx Untouched Human Classical Monocyte Cell Isolation Kit (Column-based) is used for Negative CD14+ Cell Isolation (Untouched), with the Key Features, such as Preserves native cell function; maintains surface marker integrity; ideal for functional studies

Протокол

  1. Preparation: Prepare a single-cell suspension from cryopreserved or fresh PBMCs.
  2. Labeling: Add the Biotin-Antibody Cocktail (targets non-CD14 cells: T cells, B cells, NK cells, etc.). Incubate.
  3. Bead Binding: Add Anti-Biotin Microbeads to bind the labeled non-target cells. Incubate.
  4. Separation: Apply the suspension to the separation column placed in a magnetic field.
  5. Elution: Unlabeled CD14+ Classical Monocytes pass through the column freely and are collected (Untouched).

Данные валидации

Выделение классических моноцитов высокой чистоты из мононуклеарных клеток периферической крови человека (PBMC) с использованием SOLIDEX®-ISOEx Untouched Набор для выделения классических моноцитов человека (на колонках)

Каталожный номер GM-Tg-hg-T23212-iso-kit-1
В данном эксперименте SOLIDEX®-ISOEx Untouched Набор для выделения классических моноцитов человека (на колонках) использовался для выделения и очистки классических моноцитов из криоконсервированных PBMC. Сначала немоноцитарные клетки были специфически помечены коктейлем антител, конъюгированных с биотином (Antibody Cocktail), с последующей инкубацией с анти-биотиновыми наномагнитными частицами. Чистота клеточных популяций до и после выделения анализировалась методом проточной цитометрии. Чистота CD14+ классических моноцитов в прошедшей фракции увеличилась с 21.7% до 92.1%. Результаты эксперимента показывают, что этот набор эффективно удаляет загрязняющие клетки, обеспечивая получение высокочистых, нетронутых (не связанных с антителами) классических моноцитов.

Рисунок 14. Выделение классических моноцитов высокой чистоты из мононуклеарных клеток периферической крови человека (PBMC) с использованием SOLIDEX®-ISOEx Untouched Набор для выделения классических моноцитов человека (на колонках).Результаты проточной цитометрии показывают, что чистота CD14+ классических моноцитов увеличилась с 21.7% до 92.1%.

ISOEx набор для выделения клеток & Nanobeads validation
Нажмите, чтобы увеличить

Предпосылки

Why Choose SOLIDEX®-ISOEx Untouched Human Classical Monocyte Isolation Kit?

  • True "Untouched" Isolation: Target monocytes are never bound by antibodies or beads, preventing Fc-receptor activation and preserving differentiation potential.
  • Exceptional Purity: The Column-based matrix acts as a physical filter, allowing rigorous washing of contaminants to achieve >90% purity compared to column-free methods.
  • Preserved Physiology: Gentle isolation ensures cells remain in a resting state, critical for accurate immunological studies.
  • Rapid Workflow: Isolate cells in under 45 minutes with a simple, standardized protocol.

Часто задаваемые вопросы

What are the sample requirements?
We recommend using fresh or cryopreserved Human PBMCs. For cryopreserved samples, ensure high viability (>90%) and proper DNase treatment during thawing to prevent clumping. Leukapheresis products are also compatible.
Can I stain the cells for Flow Cytometry immediately after isolation?
Yes. Since this is an "Untouched" (Negative Selection) kit, the CD14 receptors and other surface markers are free and not blocked by magnetic beads or antibodies. You can proceed directly to staining.
How does the Column-based separation work?
The column contains a ferromagnetic matrix (spheres). When placed in a magnetic field, the matrix amplifies the magnetic force by 10,000-fold, effectively retaining the magnetically labeled non-target cells while unlabeled monocytes pass through.
Are the separation columns reusable?
No. The columns are designed for single-use to ensure sterility and prevent cross-contamination between patient samples.
What is the advantage of Column-based over Column-free methods?
Column-based methods typically offer higher purity. The column allows for washing of the retained cell mass, ensuring that only the specific "Untouched" cells elute. Column-free methods often struggle with non-specific entrapment of target cells in the magnetic clump, leading to lower purity or recovery.
Is this protocol compatible with automated systems?
Yes, the SOLIDEX®-ISOEx column format is compatible with most standard automated magnetic separation instruments designed for column-based protocols.

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